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Chitinase-3-Like Protein 1 Sandwich Human ELISA Kit 2 Hours Assay Length

Categories Human ELISA Kit
Brand Name: BT Lab
Model Number: Cat.No E2063Hu
Certification: CE, ISO9001:2005, MSDS
Place of Origin: Shanghai, China
MOQ: Negotiation
Price: Negotiation
Supply Ability: Western Union, T/T
Delivery Time: 1-3 business days, bulk order within one week
Packaging Details: Wrapped with ice pack and styrofoam package
Lead Time: Within 48 hours
In Stock: Yes
OEM: Acceptable
Assay Principle: Sandwich
Sample: Serum,plasma,urine,tissue,cell culture supernatant
Storage: 2-8°C
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    Chitinase-3-Like Protein 1 Sandwich Human ELISA Kit 2 Hours Assay Length

    Human High Specificity and Sensitivity Chitinase-3-like Protein 1 ELISA Assay Kit 2 Hours Assay Length


    *This product is for research use only, not for use in diagnosis procedures. It’s highly recommend to read this instruction entirely before use.

    Precision

    Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

    Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

    CV(%) = SD/mean x 100

    Intra-Assay: CV<8%

    Inter-Assay: CV<10%


    Intended Use

    This sandwich kit is for the accurate quantitative detection of human Chitinase-3-like Protein 1 (also known as YKL-40/CHI3L1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

    Cat.No E2063Hu

    Standard Curve Range: 1ng/ml - 400ng/ml

    Sensitivity: 0.61ng/ml

    Size: 96 wells


    Reagent Provided

    ComponentsQuantity
    Standard Solution (480ng/ml)0.5ml x1
    Pre-coated ELISA Plate12 * 8 well strips x1
    Standard Diluent3ml x1
    Streptavidin-HRP6ml x1
    Stop Solution6ml x1
    Substrate Solution A6ml x1
    Substrate Solution B6ml x1
    Wash Buffer Concentrate (25x)20ml x1
    Biotinylated human YKL-40/CHI3L1 Antibody1ml x1
    User Instruction1
    Plate Sealer2 pics
    Zipper bag1 pic

    Reagent Preparation

    All reagents should be brought to room temperature before use.

    Standard Reconstitute the 120μl of the standard (480ng/ml) with 120μl of standard diluent to generate a 240ng/ml standard stock solution. Allow the standard to sit for 15 mins with gentle agitation prior to making dilutions. Prepare duplicate standard points by serially diluting the standard stock solution (240ng/ml) 1:2 with standard diluent to produce 120ng/ml, 60ng/ml, 30ng/ml and 15ng/ml solutions. Standard diluent serves as the zero standard(0 ng/ml). Any remaining solution should be frozen at -20°C and used within one month. Dilution of standard solutions suggested are as follows:


    240ng/mlStandard No.5120μl Original Standard + 120μl Standard Diluent
    120ng/mlStandard No.4120μl Standard No.5 + 120μl Standard Diluent
    60ng/mlStandard No.3120μl Standard No.4 + 120μl Standard Diluent
    30ng/mlStandard No.2120μl Standard No.3 + 120μl Standard Diluent
    15ng/mlStandard No.1120μl Standard No.2 + 120μl Standard Diluent

    Standard ConcentrationStandard No.5Standard No.4Standard No.3Standard No.2Standard No.1
    480ng/ml240ng/ml120ng/ml60ng/ml30ng/ml15ng/ml

    Wash Buffer Dilute 20ml of Wash Buffer Concentrate 25x into deionized or distilled water to yield 500 ml of 1x Wash Buffer. If crystals have formed in the concentrate, mix gently until the crystals have completely dissolved.


    Summary

    1. Prepare all reagents, samples and standards.

    2. Add sample and ELISA reagent into each well. Incubate for 1 hour at 37°C.

    3. Wash the plate 5 times.

    4. Add substrate solution A and B. Incubate for 10 minutes at 37°C.

    5. Add stop solution and color develops.

    6. Read the OD value within 10 minutes.


    Calculation of Result

    Construct a standard curve by plotting the average OD for each standard on the vertical (Y) axis against the concentration on the horizontal (X) axis and draw a best fit curve through the points on the graph. These calculations can be best performed with computer-based curve-fitting software and the best fit line can be determined by regression analysis.

    Quality Chitinase-3-Like Protein 1 Sandwich Human ELISA Kit 2 Hours Assay Length for sale
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